Journal: Molecular Medicine

Article Title: miR-351 promotes atherosclerosis in diabetes by inhibiting the ITGB3/PIK3R1/Akt pathway and induces endothelial cell injury and lipid accumulation

doi: 10.1186/s10020-022-00547-9

Figure Lengend Snippet: A mouse model of atherosclerosis was established, and pathological examination was conducted. A One-touch detection of the fasting blood glucose FBG index in mice. N = 10 mice per group. B Weight results of mice in each group. N = 10 mice per group. C Oil red O was used to stain the aorta in the thoracic region of mice in each group, and red stain indicated the site of oil presence or plaque formation. D HE staining results: The vascular tissue of the abdominal aorta is shown in the upper part, and the aortic arch is shown in the lower part. The scale bar represents 50 μm. E Masson trichromatic staining was performed on collagen, with blue representing collagen parts and red representing muscle fibers and other tissues. The scale bar represents 50 μm. Histopathological test, N = 3 mice per group

Article Snippet: After 48 h, oil red O staining was performed according to the instructions of the oil red O staining kit (cat no. G1262, Solarbio).

Techniques: Staining

Journal: Molecular Medicine

Article Title: miR-351 promotes atherosclerosis in diabetes by inhibiting the ITGB3/PIK3R1/Akt pathway and induces endothelial cell injury and lipid accumulation

doi: 10.1186/s10020-022-00547-9

Figure Lengend Snippet: Effect of miR-351 silencing on the atherosclerotic cell model. A Lentivirus was used to infect cells and deliver packaged shRNA plasmids, and the success of infection was determined by fluorescence microscopy. The scale bar represents 100 μm. B qPCR was used to detect the silencing efficiency of shRNA on the miR-351 gene in the three groups. C MiR-351 was treated with ox-LDL + 30 mM glucose for 48 h, and MTT was used to detect the activity of cells in each group. D DAPI (1 μg/ml) was used for nuclear staining of cells in each group, and apoptotic cells are shown in red circles. The scale bar represents 50 μm. E Flow cytometry was used to detect apoptosis. Annexin V-FITC fluorescence intensity is shown by the horizontal coordinates, and PI staining is shown by the vertical coordinates. Dead cells are present in the R2 quadrant, late apoptotic cells are in the R3 quadrant, normal cells are in the R4 quadrant, and early apoptotic cells are in the R5 quadrant. F Flow cytometry was used to detect apoptosis. G Oil red O stains cells with oil, and red stain indicates the oil-positive part. The scale bar represents 50 μm. **P < 0.01, *P < 0.05, vs. the control group. # P < 0.05, vs. LDL + HG group. N = 3 per group

Article Snippet: After 48 h, oil red O staining was performed according to the instructions of the oil red O staining kit (cat no. G1262, Solarbio).

Techniques: shRNA, Infection, Fluorescence, Microscopy, Activity Assay, Staining, Flow Cytometry